Clinical Performance of Check-Direct CPE, a Multiplex PCR for Direct Detection of bla KPC , bla NDM and/or bla VIM , and bla OXA-48 from Perirectal Swabs
نویسندگان
چکیده
منابع مشابه
Direct detection of bla(OXA-23) gene from endotracheal aspirates by real time PCR.
Acinetobacter baumannii has emerged worldwide as an important nosocomial pathogen. 1 The carbapenems are highly active against A. baumannii, but their activity has been threatened by the widespread of Class D carbapenemases, mainly OXA-23, which is highly prevalent in Brazil. 2 Treatment of patients infected by carbapenem-resistant A. baumannii (CRAB) has been very difficult and therapy is usua...
متن کاملPolyclonal spread of bla(OXA-23) and bla(OXA-58) in Acinetobacter baumannii isolates from Argentina.
BACKGROUND In order to study the enzymatic carbapenem resistance mechanisms in Acinetobacter baumannii isolates from Argentina, we performed molecular characterization on 41 epidemiologically unrelated strains isolated from 1995 to 2006 with diminished susceptibilities to imipenem and meropenem. METHODOLOGY Acinetobacter baumannii isolates were identified with the ARDRA technique. The total g...
متن کاملEvaluation of a new real-time PCR assay (Check-Direct CPE) for rapid detection of KPC, OXA-48, VIM, and NDM carbapenemases using spiked rectal swabs.
To prevent the spread of carbapenemase-producing bacteria, a fast and accurate detection of patients carrying these bacteria is extremely important. The Check-Direct CPE assay (Check-Points, Wageningen, The Netherlands) is a new multiplex real-time PCR assay, which has been developed to detect and differentiate between the most prevalent carbapenemase genes encountered in Enterobacteriaceae (bl...
متن کاملbla(IMP) and bla(VIM) mediated carbapenem resistance in Pseudomonas and Acinetobacter species in India.
INTRODUCTION The emergence and rapid spread of blaIMP and blaVIM metallo-beta-lactamase (MBL) producing Gram-negative bacteria causing nosocomial infections are of concern worldwide due to limited treatment options. METHODOLOGY A total of 179 nonreplicate, consecutive, carbapenem resistant Pseudomonas aeruginosa (61), Acinetobacter baumannii (116), Acinetobacter lwoffii (1) and Pseudomonas st...
متن کاملMultiplex PCR for bla(CTX-M) & bla(SHV) in the extended spectrum beta lactamase (ESBL) producing gram-negative isolates.
BACKGROUND & OBJECTIVE Phenotyping is commonly used for detection of extended spectrum beta lactamase (ESBL) production in gram-negative isolates. ESBLs are mainly coded for by three important genes, namely bla(TEM), bla(SHV) and bla(CTX-M). In this study we used a multiplex PCR as a rapid method to identify two common genes (bla(CTX-M) & bla(SHV)) responsible for extended spectrum beta lactama...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2015
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.01921-15